Hiroshima Institute of Technology

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NAKAI Tadashi

Profile Research field Research achievement

 

Reports
No.Title URL, Journal, Vol( No), Start Page- End Page, Publication date 
1
Development of a Fluorescent Protein-based Laboratory Course for Life Science Students (in press) , Bull. Hiroshima Inst. Tech. Education, 19,  61- 69, Feb. 3, 2020 
2
Crystal structure of P-protein of the glycine cleavage system from Thermus thermophilus HB8 , SPring-8 Research Frontiers 2005, ,  14- 15, Nov. 2006 

 

Academic papers
No.Title URL, Journal, Vol( No), Start Page- End Page, Publication date, DOI 
1
[Protein Cyclization Technology for Enzyme Stabilization: Mechanism and Industrial Applications of the SpyRing Method] (Article in Japanese) , Kagaku to Seibutsu, 63( 7), 306- 308, Jul. 1, 2025, 10.1271/kagakutoseibutsu.63.306 
2
Eight genes are necessary and sufficient for biogenesis of quinohemoprotein amine dehydrogenase , Biosci. Biotechnol. Biochem., 85( 9), 2026- 2029, Jun. 30, 2021, 10.1093/bbb/zbab117 
3
[Novel radical SAM enzyme forming intrapeptidyl crosslinks] (Article in Japanese) , Seikagaku, 88( 4), 506- 510, Aug. 2016, 10.14952/SEIKAGAKU.2016.880506 
4
Cellular uptake of hepatitis B virus envelope L particles is independent of sodium taurocholate cotransporting polypeptide, but dependent on heparan sulfate proteoglycan. , Virology, 497,  23- 32, Jul. 13, 2016, 10.1016/j.virol.2016.06.024 
5
Mutational analysis of hepatitis B virus pre-S1 (9-24) fusogenic peptide. , Biochemical and biophysical research communications, 474( 2), 406- 412, Apr. 2016, 10.1016/j.bbrc.2016.04.125 
6
Bio-nanocapsules displaying various immunoglobulins as an active targeting-based drug delivery system. , Acta biomaterialia, 35,  238- 247, Feb. 2016, 10.1016/j.actbio.2016.02.010 
7
Cytokine-dependent activation of JAK-STAT pathway in Saccharomyces cerevisiae. , Biotechnology and bioengineering, 113( 8), 1796- 1804, Feb. 2016, 10.1002/bit.25948 
8
Probing the Catalytic Mechanism of Copper Amine Oxidase from Arthrobacter globiformis with Halide Ions. , The Journal of biological chemistry, 290( 38), 23094- 23109, Sep. 2015, 10.1074/jbc.M115.662726 
9
The Radical S-Adenosyl-L-methionine Enzyme QhpD Catalyzes Sequential Formation of Intra-protein Sulfur-to-Methylene Carbon Thioether Bonds , J. Biol. Chem., 290( 17), 11144- 11166, Mar. 2015, 10.1074/jbc.M115.638320 
10
Identification of Genes Essential for the Biogenesis of Quinohemoprotein Amine Dehydrogenase. , Biochemistry, 53( 5), 895- 907, Jan. 2014, 10.1021/bi401625m 
11
[An unusual protease essential for biogenesis of quinohemoprotein amine dehydrogenase] (Article in Japanese) , Seisan to Gijutsu, 64( 4), 37- 39, Oct. 2012,  
12
An unusual subtilisin-like serine protease is essential for biogenesis of quinohemoprotein amine dehydrogenase. , J. Biol. Chem., 287( 9), 6530- 6538, Jan. 2012, 10.1074/jbc.M111.324756 
13
[Mechanisms of biosynthesis of built-in cofactors] (Article in Japanese) , Seikagaku, 83( 8), 691- 703, Aug. 2011,  
14
A protein-protein interaction map of trypanosome ~20S editosomes , J. Biol. Chem., 285( 8), 5282- 5295, Feb. 2010, 10.1074/jbc.M109.059378 
15
Structural bases for the specific interactions between the E2 and E3 components of the Thermus thermophilus 2-oxo acid dehydrogenase complexes , J. Biochem., 143( 6), 747- 758, Jun. 2008, 10.1093/jb/mvn033 
16
[Crystal structure of P-protein of the glycine cleavage system: Implications for nonketotic hyperglycinemia] (Article in Japanese) , Journal of the Crystallographic Society of Japan, 48( 4), 265- 270, Sep. 2006, 10.5940/jcrsj.48.265 
17
Structure of P-protein of the glycine cleavage system: Implications for nonketotic hyperglycinemia , EMBO J., 24( 8), 1523- 1536, Mar. 2005, 10.1038/sj.emboj.7600632 
18
Ligand-induced conformational changes and a reaction intermediate in branched-chain 2-oxo acid dehydrogenase (E1) from Thermus thermophilus HB8, as revealed by X-ray crystallography , J. Mol. Biol., 337( 4), 1011- 1033, Apr. 2004, 10.1016/j.jmb.2004.02.011 
19
Structure of Thermus thermophilus HB8 H-protein of the glycine-cleavage system, resolved by a six-dimensional molecular-replacement method , Acta Crystallogr. D Biol. Crystallogr., 59( 9), 1610- 1618, Sep. 2003, 10.1107/S0907444903014975 
20
Coexpression, purification, crystallization and preliminary X-ray characterization of glycine decarboxylase (P-protein) of the glycine-cleavage system from Thermus thermophilus HB8 , Acta Crystallogr. D Biol. Crystallogr., 59( 3), 554- 557, Mar. 2003, 10.1107/S090744490300043X 
21
Mechanism of preferential enrichment, an unusual enantiomeric resolution phenomenon caused by polymorphic transition during crystallization of mixed crystals composed of two enantiomers , J. Am. Chem. Soc., 124( 44), 13139- 13153, Nov. 2002, 10.1021/ja020454r 
22
Substrate recognition mechanism of thermophilic dual-substrate enzyme , J. Biochem., 130( 1), 89- 98, Jul. 2001, 10.1093/oxfordjournals.jbchem.a002966 
23
Structures of Escherichia coli histidinol-phosphate aminotransferase and its complexes with histidinol-phosphate and N-(5'-phosphopyridoxyl)-L-glutamate: double substrate recognition of the enzyme , Biochemistry, 40( 15), 4633- 4644, Apr. 2001, 10.1021/bi002769u 
24
Three-dimensional structure of 4-amino-4-deoxychorismate lyase from Escherichia coli , J. Biochem., 128( 1), 29- 38, Jul. 2000, 10.1093/oxfordjournals.jbchem.a022727 
25
Free energy requirement for domain movement of an enzyme , J. Biol. Chem., 275( 25), 18939- 18945, Jun. 23, 2000, 10.1074/jbc.275.25.18939 
26
A new host 2,3,6,7,10,11-hexahydroxy triphenylene which forms chiral inclusion crystalline lattice: X-ray structural study of the chiral crystalline lattice , J. Phys. Org. Chem., 13( 1), 39- 45, Jan. 2000, 10.1002/(SICI)1099-1395(200001)13:1<39::AID-POC203>3.0.CO;2-8 
27
Extremely Long C-C Bond in (-)-trans-1,2-Di-tert-butyl-1,2-diphenyl- and 1,1-Di-tert-butyl-2,2-diphenyl-3,8-dichlorocyclobuta[b]naphthalenes. , J. Org. Chem., 64( 9), 3102- 3105, Apr. 30, 1999, 10.1021/jo982010c 
28
Structure of Thermus thermophilus HB8 aspartate aminotransferase and its complex with maleate , Biochemistry, 38( 8), 2413- 2424, Feb. 23, 1999, 10.1021/bi9819881 
29
Preferential enrichment: Mode of polymorphic transformation of a mixed crystal into a racemic compound crystal , Mol. Cryst. Liq. Cryst., 313( 1), 211- 216, Dec. 1998, 10.1080/10587259808044277 
30
The novel substrate recognition mechanism utilized by aspartate aminotransferase of the extreme thermophile , J. Biol. Chem., 273( 45), 29554- 29564, Nov. 6, 1998, 10.1074/jbc.273.45.29554 
31
Crystallization and preliminary X-ray characterization of aspartate aminotransferase from an extreme thermophile, Thermus thermophilus HB8 , Acta Crystallogr. D Biol. Crystallogr., 54( 5), 1032- 1034, Sep. 1, 1998, 10.1107/S090744499800434X 

 

Conference Activities & Talks
No.Title, URL, Presentation date, Vol( No), Start Page- End Page, Publication date 
1
Biochemical and structural analysis of serine proteinase involved in biosynthesis of active-site subunit of quinohemoprotein amine dehydrogenase, , Mar. 28, 2020, ,  1661- 1661,   
2
Promoter analysis of Pden_1710, a gene neighboring the quinohemoprotein amine dehydrogenase operon (submitted), , Mar. 28, 2020, ,  1662- 1662,   
3
Functional analysis of serine proteinase involved in biosynthesis of active-site subunit of quinohemoprotein amine dehydrogenase, , Mar. 24, 2019, ,  639- 639,   
4
Functions of the enzyme complex involved in post-translational modification of quinohemoprotein amine dehydrogenase, , Mar. 16, 2018, ,  825- 825,   
5
Cyclic peptide formation by a peptide thioether crosslink-forming enzyme QhpD: Substrate peptide length and sequence allowed for the crosslink, , Mar. 15, 2018, ,  824- 824,   
6
FAD-dependent monooxygenase involved in quinone cofactor biosynthesis: spectroscopic properties and interaction with substrate protein QhpC, , Mar. 18, 2017, ,  847- 847,   
7
Analysis of substrate specificity of radical SAM enzyme QhpD for developing novel cyclic peptides, , Mar. 18, 2017, ,  753- 753,   
8
Purification and spectroscopic properties of FAD-dependent monooxygenase involved in quinone cofactor biosynthesis, , Sep. 27, 2016, ,  3P-375- 3P-375,   
9
Analysis of substrate specificity of intrapeptidyl thioether bond forming enzyme QhpD, , Sep. 27, 2016, ,  3P-377- 3P-377,   
10
Mechanism of sequential formation of intrapeptidyl thioether cross-links by the radical SAM enzyme QhpD, , Sep. 7, 2016, ,  OA-02- OA-02,   
11
Radical SAM superfamily enzymes catalyzing post窶稚ranslational modifications of peptides, , Dec. 1, 2015, ,  1W16-2- 1W16-2,   
12
Biogenesis process of quinohemoprotein amine dehydrogenase accompanying multi-step posttranslational modification and membrane translocation, , Oct. 17, 2014, ,  3T09p-08- 3T09p-08,   
13
Complete journey of quinohemoprotein amine dehydrogenase from genes to periplasm, , Aug. 27, 2014, ,  ST19- ST19,   
14
Analysis of Multisite Cross-linking Reaction by Intra-peptidyl Thioether Bond Forming Enzyme, , Sep. 13, 2013, ,  3T18p-07- 3T18p-07,   
15
Functional Analysis of Intra-peptide Thioether Forming Enzyme Essential for Biogenesis of Quinohemoprotein Amine Dehydrogenase, , Dec. 16, 2012, ,  3T03-08- 3T03-08,   
16
Roles of Peripheral Genes in Biogenesis of Quinohemoprotein Amine Dehydrogenase, , Sep. 24, 2011, ,  4P-0224- 4P-0224,   
17
Structure and Function of [Fe-S]-cluster-binding Protein Essential for Biogenesis of Quinohemoprotein Amine Dehydrogenase, , Sep. 24, 2011, ,  4P-0192- 4P-0192,   
18
Structural bases for the specific interactions between the E2 and E3 components of the Thermus thermophilus 2-oxo acid dehydrogenase complexes, , Oct. 23, 2009, ,  JB論文賞- JB論文賞,   
19
Crystal structure of P-protein of the glycine cleavage system, , Aug. 2005, 61,  C189- C190,   
20
X-ray structure studies of H- and L-proteins of glycine cleavage system from Thermus thermophilus, , Aug. 2002, 58,  C286- C286,   
21
Dynamism of enzyme : Induced-fit by the hydrophobicity of the substrate., , Dec. 1, 1998, ,  324- 324,   
22
A thermophilic dual-substrate enzyme has a flexible substrate-binding site, , Dec. 1, 1998, ,  325- 325,   
23
Conformational change in extremely thermophilic bacterium aspartate aminotransferase., , Sep. 7, 1998, 38( Supple 2), S40- S40,